FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_lacz1_22_3.35100000086451.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_lacz1_22_3.35100000086451.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814988
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG32950.016.4556087
GCGGTAG31400.015.69162911
ACCGCGG36000.014.1865538
CTGGACG32700.013.9736255
CGGTAGC34850.013.55720212
GGACGAC30600.013.3814237
GGCACGT35450.013.32778120
CCGCGGT37550.013.2414629
CGCGGTA37550.013.06171310
TAACCGC41600.013.0402086
AGTCTGA39350.012.92159233
CCTGGAC37100.012.37704854
TGGACGA38900.011.9779246
TGGCACG41600.011.84419519
GCACGTT41550.011.804321
TACCTGG40750.011.655092
CGTCCAG25800.011.42365710
GTCGTCC24900.011.3847848
TAGATAG32650.011.2373855
GTACCTG44000.011.1531511
Produced by FastQC (version 0.11.2)