FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_gfp_5000_27_2.35100000086437.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_gfp_5000_27_2.35100000086437.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10637641
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG16150.016.7171947
GCGGTAG16850.015.48854811
ACCGCGG19950.014.0967748
AGTCTGA21500.013.81300333
CGGTAGC18950.013.65341412
GTCGTCC15800.013.527558
TAGCTGG20150.013.17527215
CCTGGAC20200.012.4803134
GCACGTT21100.012.4756221
AGTCCTC10950.012.32791835
TCGTCCA19650.012.2510659
CGTCCAG17850.012.22604910
CCGCGGT21750.012.1026149
GGCACGT21050.012.077723520
CTGGACG21250.011.8636395
GTCTGAA25700.011.81825334
TGGCACG22900.011.69150119
GGACGAC16200.011.6659187
GTCCAGG18750.011.63919811
GTCTTAG17700.011.5735281
Produced by FastQC (version 0.11.2)