FastQCFastQC Report
Wed 5 Apr 2017
HKCHCBCXY_l01n01_foxf_22_2.351000000863f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCHCBCXY_l01n01_foxf_22_2.351000000863f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40413820
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG70350.015.1268997
CTGGACG72850.014.2439545
GGACGAC70750.014.0555767
GCGGTAG66150.013.91059611
AGTCTGA88450.013.71019833
ACCGCGG72450.013.6636468
CCTGGAC80300.012.92244154
CCGCGGT75550.012.596749
CGCGGTA80100.012.16207510
GTCTGAA100800.011.94114834
CGTCCAG55800.011.89433410
CGGTAGC76000.011.75247212
TAACCGC91750.011.7513736
TGGACGA91900.011.7321946
TACCTGG92050.011.5663852
CTCCGAG43850.011.39059545
GTCGTCC57050.011.1604868
GTATAAG200950.011.1014521
TAGATAG71150.011.0410345
GGCACGT82550.011.03814720
Produced by FastQC (version 0.11.2)