FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20990473
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16791987.9998101996081745TruSeq Adapter, Index 7 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7374583.5132986283825045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3728491.776277266357933No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG3022031.4397150554920797TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT355270.16925297490914093TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGG349970.16672801989740774TruSeq Adapter, Index 7 (97% over 36bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338270.16115406260735524No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGA263690.12562365793281552TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGG2415050.067.0135733
CGTATGC2392300.067.0118446
ACGAGTG2423650.066.9124232
GTATGCC2397350.066.8534247
GCCGTCT2372950.066.8087751
TGCCGTC2384650.066.8087350
TATGCCG2394750.066.8035548
ATGCCGT2392950.066.79613549
CACGAGT2435150.066.69591531
CTCGTAT2393350.066.6768544
TCGTATG2403650.066.6753245
GTGGATA2429050.066.55492436
AGTCACG2458800.066.5392928
GTCACGA2457600.066.4816229
GGATATC2428500.066.4618638
CCGTCTT2391500.066.32085452
CTGCTTG2339450.066.3003459
AGGGGGG877550.066.290221
GAGTGGA2439900.066.28062434
TATCTCG2421800.066.274441
Produced by FastQC (version 0.11.2)