FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10967919
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1691581.5422980421354315TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG252430.23015304908798104TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145120.13231315803845745No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139700.12737147311171793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG252600.064.95421633
CGTACGT253850.064.5132434
GTACGTA258750.063.4935635
CGTATGC257750.062.97579646
GTATGCC258300.062.89484447
CACCGTA264850.062.44489331
GCCGTCT255200.062.3587951
CTCGTAT258350.062.33545344
TATGCCG260100.062.28715548
GTCACCG267900.062.18722529
TACGTAA263800.062.14758336
ATGCCGT261050.062.04500249
TGCCGTC259950.061.9094250
TCGTATG264400.061.561545
ACCGTAC267650.061.4822932
CGTAATC264100.061.22787538
AGTCACC273850.061.13842428
TCACCGT275350.060.1530130
CTGCTTG252100.059.92347359
CCGTCTT266150.059.87098752
Produced by FastQC (version 0.11.2)