FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27866072
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4351401.5615404998594706TruSeq Adapter, Index 8 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1361960.4887520566228351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG581790.20878077111119211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG639350.066.3975646
ATGCCGT647700.064.7853547
GTATGCC665050.064.5372145
CGTATGC675350.063.34348344
TGCCGTC679200.061.70961448
TCGTATG697300.060.3501243
GTCACAC771700.059.57547429
CTCGTAT704000.059.48642342
GCCGTCT707300.058.8615249
AGTCACA806200.057.45876728
TCTCGTA742750.056.81926341
TCACACT812850.056.42742530
CAGTCAC826650.056.2043727
ACACTTG820100.055.7119432
CACACTT837000.054.7524131
AGGGGGG196300.054.427021
CCAGTCA865200.053.7484526
AAAAAGG713000.053.65435869
CACTTGA872350.052.22918333
AATCTCG827050.052.04789439
Produced by FastQC (version 0.11.2)