FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26880812
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3018001.122733941221716TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG399100.065.3328446
ATGCCGT410800.063.446947
GTATGCC419000.062.32048845
CGTATGC424500.061.52929344
TGCCGTC433700.060.16044648
TCGTATG452550.057.79943543
TATCTCG447000.057.78613739
CACGCCA448200.057.3124131
GTCACGC453050.057.00620729
TCACGCC451150.056.96260530
ACGCCAA453100.056.7611332
CTCGTAT459150.056.6604442
GCCGTCT463650.056.23606549
CGCCAAT462950.055.18116833
GCCAATA465250.054.8952834
TCTCGTA489450.053.25863341
AGTCACG493350.053.12629328
CCAATAT507200.050.49844735
CAGTCAC525850.050.11202227
ATATCTC518950.049.78100238
Produced by FastQC (version 0.11.2)