FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25070650
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4344771.733010512292262TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG586900.065.2074246
ATGCCGT601200.063.71965847
GTATGCC603900.063.38323245
CGTATGC620150.061.80026244
ACTGACC613300.061.49790232
TGCCGTC628400.060.92315748
TCGTATG648050.059.2297743
CTCGTAT638250.058.5068242
GCCGTCT653550.058.45026449
CTGACCA646100.058.3020733
GACCAAT646200.058.17872635
AGTCACT663850.058.16480328
ACCAATC631500.058.0318936
CACTGAC666600.057.0828831
TGACCAA664850.056.51458434
TCTCGTA669400.055.79993441
GTCACTG694200.055.41777829
CAGTCAC699750.055.3928327
CCAATCT677300.054.48962437
TCACTGA710550.053.8799730
Produced by FastQC (version 0.11.2)