FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30488338
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG13832044.5368297871796095TruSeq Adapter, Index 2 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2267680.7437860338598975No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1820110.5969856408702895TruSeq Adapter, Index 2 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1075250.3526758329693144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1856250.067.4833646
ATGCCGT1859400.067.07773647
GTATGCC1882100.066.7672845
TGCCGTC1885300.065.9662448
TATCTCG1896500.065.9487639
CGTATGC1912100.065.6150944
CTCGTAT1908450.065.12405442
GCCGTCT1903250.065.0308449
TCGTATG1921550.064.9401943
TGTATCT1926150.064.8835437
GTATCTC1932500.064.7398538
ATGTATC1953850.064.65056636
GTCACCG2002400.064.1408929
ACCGATG1993600.064.0097932
AGTCACC2013350.064.00848428
TCACCGA2013400.063.73360430
TCTCGTA1958850.063.73189541
CCGATGT2000450.063.630833
CACCGAT2010750.063.59615731
CGATGTA2005550.063.4725834
Produced by FastQC (version 0.11.2)