FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10925821
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA429250.3928766543035988TruSeq Adapter, Index 21 (97% over 40bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG252690.23127781427134858No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193350.17696610625416614No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA144300.13207245478394714TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC99900.058.01722746
GTATGCC101250.057.82291847
AGTCACG147200.057.63361428
GTCACGT148050.056.64996729
TATGCCG100650.055.73396748
GCCGTCT96800.055.45306851
ATGCCGT103300.055.1376249
TGCCGTC103150.055.0171350
CAGTCAC156750.054.8486127
CTCGTAT118350.054.56960344
CGTTTCG152150.054.2819533
TCGTATG114000.054.2005845
CGGAATC149350.053.87092238
CCAGTCA160200.053.66888426
GTTTCGG154550.053.28470234
TCCAGTC165550.052.01761625
ACTCCAG166900.051.61493723
GAACTCC173300.050.00945721
TCACGTT167800.049.8998230
GGAATCT149050.049.65081839
Produced by FastQC (version 0.11.2)