FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12210618
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1703691.3952528856442812TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG260740.21353546560870218TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139990.11464612192437762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA252450.064.983536
GCCTTAT261900.062.70459737
CGTATGC263500.062.55620246
CGTGGCC265050.062.09900733
GTATGCC267150.061.7787447
TATGCCG267000.061.51626648
ATGCCGT267600.061.19684649
CTCGTAT267600.061.12074744
TGGCCTT268350.061.12005635
GCCGTCT265750.060.9608151
TGCCGTC268550.060.86499850
TCGTATG271850.060.5586145
GTGGCCT271800.060.36790534
GTCACGT280850.059.62621729
AGTCACG282800.059.54445628
CCTTATC276500.058.93194238
TATCTCG277350.058.5625841
CTTATCT278000.058.1671939
CCGTCTT279200.058.09826352
CTGCTTG267150.057.81307259
Produced by FastQC (version 0.11.2)