FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11663227
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3694933.168016879033564TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG605270.518955860157742TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170320.14603162572416709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC548000.065.55595446
ATGCCGT548200.065.3987649
GTATGCC548950.065.360847
GCCGTCT543850.065.3056951
TGCCGTC547100.065.22784450
TATGCCG550100.065.1865448
CTCGTAT545350.065.052144
TCGTATG554250.064.8353945
ACGATCT537150.064.6653939
CGATCTC537550.064.6301140
AGTCACG576000.064.47780628
GATCTCG546800.064.45521541
GTCACGT574200.064.2713629
AACGATC543750.064.0708338
CCGTCTT557250.063.77891552
CTGCTTG546550.063.60594659
CACGTGA580950.063.30270431
TCTCGTA561700.063.25574543
CAGTCAC594550.063.19170427
CCAGTCA594600.063.1341726
Produced by FastQC (version 0.11.2)