FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10318023
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6020165.834606106227908TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1119801.0852854272567527TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGG146970.14244007791027408TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGA119300.11562292505066134TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC918300.065.62009433
GTCCGCA917550.065.56283634
ACGTCCG924250.065.50651632
CGTATGC931750.065.4957846
TCCGCAC917700.065.3851735
CGCACAT913600.065.3346437
TATGCCG932200.065.2680148
GTATGCC934800.065.2599447
GCCGTCT926950.065.15195551
AGTCACG952400.065.1356328
ATGCCGT933900.065.0999249
TGCCGTC932100.065.0479950
CACGTCC934350.065.04272531
CCGCACA913950.065.01517536
TCGTATG938750.065.00004645
CTCGTAT933050.064.9608344
GTCACGT951250.064.95261429
TCACGTC947950.064.7856730
CTGCTTG906250.064.68632559
GCACATC923950.064.67744438
Produced by FastQC (version 0.11.2)