FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12325667
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2731152.215823289725416TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG572490.4644697929937585TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG427550.065.7487635
GTCCCGA422400.065.31760436
CCGTCCC430650.065.2573334
CACCCGT437800.064.7888931
CCCGTCC436350.064.6955833
CCCGATC421100.064.68355638
ACCCGTC438300.064.6197432
CGTATGC442050.064.612446
GTATGCC441650.064.56937447
TATGCCG441500.064.50535648
TCACCCG441450.064.35766630
ATGCCGT442450.064.3023949
GTCACCC443750.064.23505429
TGCCGTC442500.064.08488550
GCCGTCT441400.063.97859651
CTCGTAT440950.063.60000644
TCGTATG448450.063.42103645
CCGATCT429100.063.2203339
AGTCACC457700.063.18231228
CGATCTC431100.063.08683440
Produced by FastQC (version 0.11.2)