FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13514846
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12048928.91532171361775TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA2991192.2132623634779116TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGGCGTCTTCTGCGTGAAAAAAAAAA219960.16275435176989808TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAG155640.11516224454203917TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG151880.11238011887075887TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGGCGTCTTCTGCTTGAAAAAAAAAA141440.1046552805707146TruSeq Adapter, Index 15 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139010.10285725786294568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGTG537800.066.08279459
CTCGTAT2277100.066.0760444
CGTATGC2135650.066.0276246
GTATGCC2142300.066.02556647
ATGCCGT2074700.066.0051549
TATGCCG2067950.065.9637348
CAGTCAC2679500.065.9170827
TCGTATG2227800.065.91225445
TCCAGTC2674250.065.899925
TGCGTGA585250.065.8945160
TGCCGTC2078350.065.8942150
GCCGTCT1998300.065.8535551
ACTCCAG2682900.065.84869423
GCGTGAA626800.065.8356161
CCAGTCA2680700.065.7969226
ATGTCAG2632950.065.79410634
GAACTCC2699300.065.7801821
ACGTCTG2718500.065.770115
CGTCTGA2718050.065.7658516
TCTCGTA2308800.065.717243
Produced by FastQC (version 0.11.2)