FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14684910
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2665081.814842583304903TruSeq Adapter, Index 14 (97% over 44bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG851920.5801329391872336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG496840.3383337044626082No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG227210.15472345421252157TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC400000.063.669746
TATGCCG398150.063.3589948
GTATGCC402950.063.2720747
ATGCCGT400850.062.90361849
GCCGTCT394250.062.8886951
TGCCGTC402200.062.5981350
CGTATCT413000.062.1881539
CTCGTAT408950.062.14973444
TCGTATG410150.062.11930545
CCGTATC422450.061.7852738
GTATCTC421850.060.74446540
TCCGTAT434400.060.53059837
CCGTCTT411900.060.3446552
GTTCCGT435300.059.95263335
TATCTCG431350.059.53015541
AGTTCCG439500.059.36198434
TCTCGTA431950.059.0794643
TTCCGTA446400.058.68701636
CTGCTTG386900.058.5985159
GTCACAG455350.058.39911729