FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12946468
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1698391.311855866789305TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG271260.20952432740728977TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC262150.061.02342646
GTATGCC263350.060.64032447
TATGCCG263900.060.435548
ATGCCGT264300.060.34310549
GCCGTCT264950.059.84327751
TGCCGTC265950.059.80009550
TCGTATG270250.059.21994445
CTCGTAT267150.059.1064744
CACAGTC278850.057.98650431
GTCACAG284900.057.3625829
CCGTCTT279300.056.85508352
CAGTCAC292550.056.66514627
CTGCTTG276300.055.98571459
ACAGTCA289450.055.7429232
AGTCAAC288150.055.5781734
CCAGTCA299700.055.31391526
TCTCGTA286800.055.1524143
AGTCACA301550.054.5959628
ACTCCAG305750.054.30694623
TCCAGTC305800.054.16519525
Produced by FastQC (version 0.11.2)