Basic Statistics
Measure | Value |
---|---|
Filename | HKCC7BGX2_n01_las32.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 23136877 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 2487971 | 10.753270633716037 | TruSeq Adapter, Index 12 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG | 307215 | 1.3278153313431196 | TruSeq Adapter, Index 12 (100% over 63bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 29970 | 0.12953347160898163 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG | 27630 | 0.11941974709897105 | TruSeq Adapter, Index 12 (100% over 63bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 335055 | 0.0 | 66.95627 | 44 |
GTATGCC | 335610 | 0.0 | 66.85274 | 45 |
TATGCCG | 335485 | 0.0 | 66.82712 | 46 |
ATGCCGT | 335190 | 0.0 | 66.81094 | 47 |
CTCGTAT | 331890 | 0.0 | 66.75964 | 42 |
TCGTATG | 335995 | 0.0 | 66.71629 | 43 |
TGCCGTC | 334275 | 0.0 | 66.70601 | 48 |
CTGCTTG | 330085 | 0.0 | 66.69016 | 57 |
GCCGTCT | 333320 | 0.0 | 66.65174 | 49 |
CCTTGTA | 335765 | 0.0 | 66.51217 | 33 |
AGTCACC | 340800 | 0.0 | 66.45345 | 28 |
GTCACCT | 339070 | 0.0 | 66.416176 | 29 |
CACCTTG | 337900 | 0.0 | 66.328255 | 31 |
TGTAATC | 333555 | 0.0 | 66.31681 | 36 |
CCGTCTT | 335005 | 0.0 | 66.312515 | 50 |
GTAATCT | 333890 | 0.0 | 66.31213 | 37 |
ACCTTGT | 337365 | 0.0 | 66.29013 | 32 |
TCTCGTA | 335105 | 0.0 | 66.191216 | 41 |
CCAGTCA | 344450 | 0.0 | 66.143326 | 26 |
CAGTCAC | 344875 | 0.0 | 66.135704 | 27 |