FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23136877
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG248797110.753270633716037TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3072151.3278153313431196TruSeq Adapter, Index 12 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG299700.12953347160898163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG276300.11941974709897105TruSeq Adapter, Index 12 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3350550.066.9562744
GTATGCC3356100.066.8527445
TATGCCG3354850.066.8271246
ATGCCGT3351900.066.8109447
CTCGTAT3318900.066.7596442
TCGTATG3359950.066.7162943
TGCCGTC3342750.066.7060148
CTGCTTG3300850.066.6901657
GCCGTCT3333200.066.6517449
CCTTGTA3357650.066.5121733
AGTCACC3408000.066.4534528
GTCACCT3390700.066.41617629
CACCTTG3379000.066.32825531
TGTAATC3335550.066.3168136
CCGTCTT3350050.066.31251550
GTAATCT3338900.066.3121337
ACCTTGT3373650.066.2901332
TCTCGTA3351050.066.19121641
CCAGTCA3444500.066.14332626
CAGTCAC3448750.066.13570427