FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21244851
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG10400104.8953508781963215TruSeq Adapter, Index 11 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1182820.5567560817442306No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1152300.5423902478770033TruSeq Adapter, Index 11 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG735710.34630038120766293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1409200.066.9134244
TATGCCG1410550.066.7613346
GTATGCC1413200.066.68252645
CGGCTAC1401750.066.45592533
ATGCCGT1416200.066.4366747
ACGGCTA1409800.066.43169432
CTCGTAT1410250.066.36928642
GCCGTCT1390700.066.2806749
GGCTACA1402750.066.2653134
TCGTATG1424350.066.2281943
CACGGCT1416700.066.18410531
TGCCGTC1405700.066.17210448
GTCACGG1428250.066.0037129
TCACGGC1426650.065.855130
GCTACAT1411850.065.8399135
AGTCACG1438700.065.7859828
CTACATC1413450.065.3926936
TCTCGTA1433850.065.2865241
CTGCTTG1394250.065.24991657
CCGTCTT1411250.065.2055250
Produced by FastQC (version 0.11.2)