FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23068113
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG306224713.274804922275177TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG4284951.8575208123872118TruSeq Adapter, Index 10 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG871170.37765117588941927No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG540350.23424109288869877No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG456610.19793990084928056TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG323130.14007647699662298TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG294160.12751801588625822TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG286490.12419307985876435TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTT266400.11548408836041335TruSeq Adapter, Index 10 (100% over 62bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG4260700.067.07545557
TGCTTGA4290300.066.63116558
GCCGTCT4341900.066.541449
CGTATGC4523750.066.53969644
GTATGCC4547350.066.463745
TATGCCG4506000.066.4565146
ATGCCGT4444850.066.4563347
TCGTATG4450300.066.43928543
TGCCGTC4399100.066.3806348
CTCGTAT4481450.066.3745642
CCGTCTT4413650.066.2889950
GCTTGAA4372550.066.23003459
TCTGCTT4383850.066.0611756
TCTCGTA4532000.066.0445241
TATCTCG4611850.065.9369739
CTTATCT4631850.065.885437
GCTTATC4763700.065.87311636
CTTCTGC4433950.065.8694254
ATCTCGT4587350.065.8055440
TAGCTTA4834500.065.71924634
Produced by FastQC (version 0.11.2)