FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16581305
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG13462848.119288560218873TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1484070.8950260549456148TruSeq Adapter, Index 9 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236250.14247973847655537No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221580.1336324251921064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1755400.067.3898144
GTATGCC1758100.067.2451145
TATGCCG1759000.067.1910946
ATGCCGT1761250.067.09350647
CTCGTAT1732350.067.03007542
TCGTATG1766350.067.0091543
GCCGTCT1756450.066.9657249
AGTCACG1770200.066.9517928
TGCCGTC1763050.066.8843248
CTGCTTG1750250.066.7842657
GATCTCG1734300.066.7544539
GTCACGA1773400.066.69849429
CACGATC1763250.066.6407431
CGATCAG1759650.066.6102833
CCGTCTT1766700.066.4524250
CAGATCT1735400.066.42187537
CAGTCAC1792500.066.3388827
AGATCTC1742700.066.3198538
ACGATCA1771750.066.2836432
CCAGTCA1795450.066.1980126
Produced by FastQC (version 0.11.2)