FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18367970
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG299215516.290069071323614TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG4071782.216782801801179TruSeq Adapter, Index 7 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412600.22463015782364626No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG410160.22330175844146086No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG328910.1790671478666396TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG281590.15330491066786367TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGATCGGAAGAGCACACGTCTGAACTCCAGTCACC210380.11453633689514954TruSeq Adapter, Index 7 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG191760.10439912521634127TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG187500.10207987055727988TruSeq Adapter, Index 7 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG4094300.067.26945557
CGTATGC4312050.067.0945744
GCCGTCT4176100.067.0737149
CTCGTAT4259350.067.0436542
TCGTATG4274250.067.0428443
GTATGCC4321850.067.0126745
TATGCCG4305500.067.0033446
ATGCCGT4274500.066.9929147
TGCCGTC4223100.066.9921948
TGCTTGA4112200.066.986758
CCGTCTT4192950.066.874150
CATCTCG4314000.066.75951439
TCTCGTA4292900.066.74113541
ATCTCGT4329350.066.5736340
GCTTGAA4186800.066.5559659
CTTCTGC4182450.066.5386554
TCTGCTT4162150.066.51617456
CGTCTTC4202650.066.4031751
GATCATC4410300.066.3727436
GTCTTCT4164600.066.2901952
Produced by FastQC (version 0.11.2)