FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15706356
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG176306111.225143502413927TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2262121.440257689307437TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG575430.36636760302644356TruSeq Adapter, Index 5 (98% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396970.2527448123549473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG388150.24712925136804487No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG171720.10933153431642577TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2422250.067.17081542
CGTATGC2541750.067.1524344
TCGTATG2529800.067.08758543
GTATGCC2549500.067.06872645
GCCGTCT2488950.067.0030149
TATGCCG2544800.066.9578546
ATGCCGT2537100.066.9516647
TGCCGTC2512500.066.891848
CTGCTTG2450650.066.7699357
CCGTCTT2502700.066.75619550
TCTCGTA2446000.066.7317941
GATCTCG2458650.066.6599739
AGTGATC2479300.066.5055336
ATCTCGT2460250.066.3754340
GTGATCT2469100.066.3105737
CACAGTG2610600.066.2626933
TGCTTGA2475550.066.2544158
CTTCTGC2494100.066.2070154
TGATCTC2471350.066.18877438
TCTGCTT2488450.066.08839456