Basic Statistics
Measure | Value |
---|---|
Filename | HKCC7BGX2_n01_las27.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15706356 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 1763061 | 11.225143502413927 | TruSeq Adapter, Index 5 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG | 226212 | 1.440257689307437 | TruSeq Adapter, Index 5 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 57543 | 0.36636760302644356 | TruSeq Adapter, Index 5 (98% over 63bp) |
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 39697 | 0.2527448123549473 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 38815 | 0.24712925136804487 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG | 17172 | 0.10933153431642577 | TruSeq Adapter, Index 5 (100% over 63bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 242225 | 0.0 | 67.170815 | 42 |
CGTATGC | 254175 | 0.0 | 67.15243 | 44 |
TCGTATG | 252980 | 0.0 | 67.087585 | 43 |
GTATGCC | 254950 | 0.0 | 67.068726 | 45 |
GCCGTCT | 248895 | 0.0 | 67.00301 | 49 |
TATGCCG | 254480 | 0.0 | 66.95785 | 46 |
ATGCCGT | 253710 | 0.0 | 66.95166 | 47 |
TGCCGTC | 251250 | 0.0 | 66.8918 | 48 |
CTGCTTG | 245065 | 0.0 | 66.76993 | 57 |
CCGTCTT | 250270 | 0.0 | 66.756195 | 50 |
TCTCGTA | 244600 | 0.0 | 66.73179 | 41 |
GATCTCG | 245865 | 0.0 | 66.65997 | 39 |
AGTGATC | 247930 | 0.0 | 66.50553 | 36 |
ATCTCGT | 246025 | 0.0 | 66.37543 | 40 |
GTGATCT | 246910 | 0.0 | 66.31057 | 37 |
CACAGTG | 261060 | 0.0 | 66.26269 | 33 |
TGCTTGA | 247555 | 0.0 | 66.25441 | 58 |
CTTCTGC | 249410 | 0.0 | 66.20701 | 54 |
TGATCTC | 247135 | 0.0 | 66.188774 | 38 |
TCTGCTT | 248845 | 0.0 | 66.088394 | 56 |