FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15101882
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG200922613.304474237052045TruSeq Adapter, Index 3 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6174684.088682456928216No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2504651.6585019006240413TruSeq Adapter, Index 3 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2295401.5199430110763679No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG227610.15071631469508237No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG199700.13223517439746912TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2714500.067.5600844
GCCGTCT2649800.067.5054149
GTATGCC2720500.067.5036545
CTCGTAT2696250.067.4784742
TCGTATG2709100.067.4604343
TATGCCG2716700.067.4367946
TGCCGTC2678500.067.3797648
ATGCCGT2706150.067.3746147
AGGCATC2723800.067.295536
GGCATCT2707950.067.2855337
CTGCTTG2624500.067.2358257
CCGTCTT2664450.067.2171150
GCATCTC2712900.067.09663438
ACTTAGG2766350.067.0717532
TCTCGTA2718600.067.0425641
CATCTCG2719450.067.03651439
CACTTAG2772750.067.02891531
ATCTCGT2730600.066.8322740
TGCTTGA2643900.066.8233658
TTAGGCA2766900.066.77128634