FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_las25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_las25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21224863
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG350691916.5226932206818TruSeq Adapter, Index 1 (100% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4462722.1025907210802726No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG3695651.7411890950721332TruSeq Adapter, Index 1 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1929130.9089010374295466No Hit
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTT570930.2689911355376004TruSeq Adapter, Index 1 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG363930.17146400426707112TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG317650.14965938767190157TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG270590.12748727753861122TruSeq Adapter, Index 1 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG4572850.067.2259957
TGCTTGA4588950.066.9886358
GCTTGAA4627450.066.7166659
CGTATGC4760500.066.5875244
GTATGCC4762200.066.5391245
GCCGTCT4692300.066.5170949
CTCGTAT4698150.066.490942
TATGCCG4767550.066.46300546
TCGTATG4774800.066.4263943
ATGCCGT4771600.066.39574447
CCGTCTT4694250.066.3446750
TGCCGTC4740950.066.3352648
TCTCGTA4712900.066.146541
ACGATCT4689650.066.1077437
GATCTCG4721250.066.0701139
CGATCTC4702500.066.0481538
CACGATC4687800.065.97780636
TCTGCTT4659850.065.9614156
CTTCTGC4666500.065.96133454
ATCTCGT4719700.065.9587640
Produced by FastQC (version 0.11.2)