FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_dj02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_dj02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9869115
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTG582076058.979553891103706No Hit
TCAAAGCCTAGTTTCGAAAGCTTTCAAGAAATCCGCGTTAGCGTAGTAAAACAATTTAGGAAAAAAAATACTAGC904750.916748867552967No Hit
GCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTG807710.8184219152375872No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTGTACTAAAATTTTGTGGCGAAATTTGAATACACATCTCATACACGCGAA366530.3713909504550307No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCACCCATGGAAATAAATTTCAAACTG355350.3600626803923148No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAGAAATAGTACTAAAATTTTGTGGCGAAATTTGAATACACA337880.34236099184172036No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTCTCCAAGCAACATCAACTGTCTCCGGTTCCACTCCAGCGATTTCACGTTC211770.21457851083911778No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGAGCAGTTACAGAAAACCTACAGTACTAAAATTTTGTGGCGAAATTTGAAT203140.20583405908229865No Hit
TCAAAGCCTAGTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTGG186420.18889231709226206No Hit
TCAAAGCCTAGTTTCGAAAGCTTACAACATCCAGGATTCCCAGGCCGTCTCCGATCCAAGTACTAAAATTTTGTG181550.1839577307590397No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCGCCACCGATGATACAAGGCGTTCGCCAGTACTAAAATTTTGTGGCGAAA168730.17096771088390397No Hit
TCAAAGCCTAGTTTCGAAAGCTCTAAAATTTTGTGGCGAAATTTGAATACACATCTCATACACGCGAACCGCAAT165160.16735036525564856No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAGACTCAAGCGCCTCGACGCGAATCATCAGCAATATCTATGGATCGGCGCA154980.1570353572736765No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAACCTG128110.1298090051640902No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTCCGTCCTCTCTGAGCTCGCCTTAGGA124550.1262017921566422No Hit
TCAAAGCCTAGTTTCGAAAGCTTGAAATATAACTAAAAAATATAATAACATTACACTGCAATTCTAGGTTTACTT117290.11884550945044212No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTACTAAAATTTTGTGGCGAAATTTGAATACACATCTCATACACGCGAACCG99030.10034334385606003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAGC9603150.068.913281
CAAAGCC9780450.068.8927462
GCCTAGT9745550.068.879556
CCTAGTT9748650.068.85437
AGCCTAG9759150.068.8221055
AAAGCCT9777350.068.818253
CTAGTTT9724350.068.777668
TAGTTTC9723550.068.709879
GTTTCGA9723500.068.7038511
AGTTTCG9723100.068.6992110
CGAAAGC9758850.068.41041615
TTTCGAA9771450.068.3751812
GAAAGCT9765100.068.33652516
TAGTCCA6601150.068.32725538
GTCCAGT6593200.068.3248740
AGTCCAG6604600.068.3239539
TCGAAAG9770800.068.31914
AGTTAGC6583650.068.31116544
CCAGTTA6581900.068.3052242
GTTAGCA6571900.068.2994445
Produced by FastQC (version 0.11.2)