FastQCFastQC Report
Thu 25 May 2017
HKCC7BGX2_n01_dj01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKCC7BGX2_n01_dj01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9592388
Sequences flagged as poor quality0
Sequence length75
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTG469069348.900159167873525No Hit
GCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTG662560.6907143455831853No Hit
TCAAAGCCTAGTTTCGAAAGCTTTCAAGAAATCCGCGTTAGCGTAGTAAAACAATTTAGGAAAAAAAATACTAGC659090.6870968939121311No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTGTACTAAAATTTTGTGGCGAAATTTGAATACACATCTCATACACGCGAA576580.6010807736300908No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAGAAATAGTACTAAAATTTTGTGGCGAAATTTGAATACACA399780.4167679622634114No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCACCCATGGAAATAAATTTCAAACTG291760.3041578384860996No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTCTCCAAGCAACATCAACTGTCTCCGGTTCCACTCCAGCGATTTCACGTTC226230.23584325404685463No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGAGCAGTTACAGAAAACCTACAGTACTAAAATTTTGTGGCGAAATTTGAAT225290.23486331036651145No Hit
TCAAAGCCTAGTTTCGAAAGCTTACAACATCCAGGATTCCCAGGCCGTCTCCGATCCAAGTACTAAAATTTTGTG196650.2050063029143525No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTACTAAAATTTTGTGGCGAAATTTGAATACACATCTCATACACGCGAACCG150310.1566971644599864No Hit
TCAAAGCCTAGTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAAACTGG150120.15649909073736382No Hit
TCAAAGCCTAGTTTCGAAAGCTTGCTTGATCTTGACTTTCAGTACTAAAATTTTGTGGCGAAATTTGAATACACA132830.13847438197871062No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAGACTCAAGCGCCTCGACGCGAATCATCAGCAATATCTATGGATCGGCGCA130390.13593069838292612No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCGCCACCGATGATACAAGGCGTTCGCCAGTACTAAAATTTTGTGGCGAAA127260.13266769442603865No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGCAACCATGGAAATAAATTTCAACCTG106670.1112027578534146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAGC9300350.068.894961
CAAAGCC9464300.068.884072
GCCTAGT9429250.068.878096
CCTAGTT9436600.068.83427
AGCCTAG9443300.068.819095
AAAGCCT9463700.068.785263
CTAGTTT9418500.068.739398
AGTTTCG9417700.068.6855110
GTTTCGA9420050.068.6779111
TAGTTTC9428200.068.607559
CGAAAGC9383950.068.4178215
GAAAGCT9382050.068.3311816
TCGAAAG9398500.068.3229314
TTTCGAA9475450.068.2807312
AAAGCTT9371800.068.2560617
TAGTCCA5348900.068.19964638
AAGCTTT6723650.068.19527418
AGTCCAG5354600.068.190239
AAGCCTA9538250.068.1864854
AGTTAGC5336850.068.181844
Produced by FastQC (version 0.11.2)