FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w5_ov.351000000aca8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w5_ov.351000000aca8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6179922
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT109990.17797959262269006TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCAG47650.012.95156334-35
CATGTCA45650.012.89503932-33
CGGGAAT28800.012.3927991
GTCAGAA49850.012.29514536-37
CTAGACT27800.012.1287768
TCTAGAC28400.012.0397537
TCACATG51250.011.763906530-31
AGTCACA53500.011.71283128-29
CTCGGAT31700.011.7094361
CGGGGAT19350.011.5589281
ACATGTC55150.011.53455932-33
CCAGTCA54500.011.36725926-27
ATTCTAG37400.011.1812095
CAGTCAC53550.010.99268926-27
TGTCAGA54700.010.93515534-35
GGGGGAT9601.8189894E-1210.9056641
TATGCCG59150.010.75453948-49
CTCGTAT57200.010.74493144-45
CCGGGAT21450.010.4272841
GTATGCC57600.010.41121846-47
Produced by FastQC (version 0.11.2)