FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w5_fb.351000000aca62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w5_fb.351000000aca62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500573
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT76420.1698006009457018TruSeq Adapter, Index 14 (97% over 44bp)
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC68590.15240281626361799No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT49140.10918609697031911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC38550.019.5870117
CTAGACT41400.017.7797978
GGTCATA13600.017.1196164
CATTCTA50450.015.9170424
ATTCTAG51600.015.3789875
TTCTAGA54600.015.0469656
TAGACTT49150.014.9762699
CCGTATC27500.014.71890438-39
CTTTCGT44800.013.8052221
TCCGTAT30450.012.87577936-37
CTGGAAT25850.012.5148731
TTCCGTA31750.012.42342236-37
TCATTCT68500.012.2084053
GTTCCGT33700.011.90331334-35
CGTATCT34050.011.67773538-39
AGTTCCG35600.011.40137234-35
CCGGGGG4609.371799E-611.3766111
CGTACAA43300.011.30343155
GCTACCT37200.011.26494238-39
TAATCCC20350.011.2013999
Produced by FastQC (version 0.11.2)