FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w5_br.351000000aca49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w5_br.351000000aca49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4608050
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT95820.20794045203502567TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC23950.014.6739467
CTCTTAT32900.014.1465628
CTAGACT25300.013.5155178
ATTCTAG30400.013.4434175
CATTCTA31450.012.8430724
GGGGATA12400.012.641342
TCTCTTA40550.011.9462077
CTCGGAT25150.011.731591
CGGGGAT17450.011.726711
AGTCAAC39700.011.59906134-35
TTCTAGA33750.011.2573696
CTCGAAT35350.010.9043251
CTGGAAT21850.010.8898461
GTATGCC46200.010.86792846-47
CTTTTCT81200.010.7836291
CCAGTCA45800.010.77965226-27
GTCACAG44450.010.6264528-29
GGTCATA12150.010.5598594
TATGCCG47750.010.48856348-49
TCGTATG49750.010.43848944-45
Produced by FastQC (version 0.11.2)