FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w4_ov.351000000aca2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w4_ov.351000000aca2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300259
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAT10600.013.020551
CTAGACT21600.012.77942851
TCTAGAC20350.012.6014617
CGGGGAT13200.012.2586361
GGTCATA10300.011.994594
CTCGGGT10700.011.5644971
CTCGAAT34450.011.3282241
CTGGAAT29000.011.1595871
CTCGGAT23150.011.1014571
CTCATAT22500.010.78760051
CTGGGTA10800.010.5760781
CTCAGCT19850.010.5494391
CTCCCTT20450.010.4726451
CTGGGTT7904.8712536E-910.2413921
CGGGAAT20000.010.2323561
CTAGGTT7955.4042175E-910.1769811
CTCTTAT29550.010.1245248
CGGGGCT7101.1604061E-710.0547231
CATTCTA30050.09.9619734
ATTCTAG28200.09.9418535
Produced by FastQC (version 0.11.2)