FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w4_fb.351000000aca12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w4_fb.351000000aca12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4074871
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC51950.1274886984152382TruSeq Adapter, Index 11 (100% over 50bp)
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT43740.10734082134133817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA12800.019.3019434
TCTAGAC28400.017.3903927
CTAGACT30200.016.0393818
GTCATAA17950.014.8231975
CATTCTA36400.014.6192244
CTGGAAT26650.014.4602971
TAATCCC18750.014.1834269
ATTCTAG38950.014.1504055
TAGACTT34650.013.9794899
CTGGGAT20650.013.8235811
CGGGGAG11700.013.4189171
CGGGTCT10600.013.0161191
CTGGGTT6953.6379788E-1213.0064331
CCGGGGG4404.0410305E-712.9753151
CGGGGCT7651.8189894E-1212.4382111
TTCTAGA44300.012.4350826
CGGGGAT14600.012.3828351
CTACGAT24650.012.3524311
CCCGGGT6201.2150849E-912.2777181
CTCGGTT15350.012.0877531
Produced by FastQC (version 0.11.2)