FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w4_br.351000000ac9f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w4_br.351000000ac9f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585533
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC38700.10793374374186487TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT25800.017.4866458
CTGGGAT12300.016.637491
TCTCTTA32000.015.1374537
ATTCTAG23550.013.9142818
TCTAGAC20550.013.8657077
CGGGGAT11650.013.48071
CATTCTA26900.013.4172627
CTTTTCT62500.013.2493221
CTAGACT21400.012.6492188
CTGGTAT12900.012.5433521
TTCTAGA26350.012.2554969
CTGGGGT4801.1543289E-611.8977391
CGGGGTT6502.5429472E-911.7146971
ACTAGCT17400.011.45959832-33
CACTAGC16900.011.3771830-31
CTGGGTT5504.853009E-711.2487721
CCCAATT15750.011.1800971
TCTTATT44000.010.6852629
CTCAGAT16550.010.6396691
TAGCTTA18400.010.57877434-35
Produced by FastQC (version 0.11.2)