FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w3_ov.351000000ac9dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w3_ov.351000000ac9dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4427654
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC566251.278893969582989TruSeq Adapter, Index 9 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT103350.029.72683542-43
GTATGCC107500.028.41675444-45
TATGCCG108850.028.15041246-47
TCTCGTA109050.028.08809540-41
CAGATCT106000.028.0100536-37
TCGTATG109500.027.99199742-43
TGCTTGA105750.027.94738458-59
CGTATGC111150.027.7620744-45
ATGCCGT110150.027.66699246-47
CCGTCTT109950.027.33305750-51
GCTTGAA108300.027.24550658-59
GCCGTCT112850.026.60901848-49
GATCAGA112400.026.54689834-35
CTTGAAA114050.026.24697960-61
AGATCTC115700.026.08541938-39
ATCAGAT115950.025.81601334-35
AGTCACG119750.025.29414628-29
CTGCTTG118650.024.98874556-57
CAGTCAC122100.024.59346226-27
TGCCGTC125250.024.54324348-49
Produced by FastQC (version 0.11.2)