FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w3_fb.351000000ac9b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w3_fb.351000000ac9b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5108401
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC633921.2409362538297208TruSeq Adapter, Index 8 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT116650.028.55832542-43
ATGCCGT118000.028.41255646-47
CGTATGC119500.027.96607844-45
TCGTATG120300.027.90973542-43
TCTCGTA120250.027.82252740-41
GCCGTCT120850.027.28152748-49
GTATGCC126500.027.0587744-45
CCGTCTT121850.027.03868150-51
TGCTTGA120600.026.74867258-59
TGAATCT123200.026.64818436-37
GCTTGAA123250.026.57799558-59
CTGCTTG122200.026.4556856-57
TATGCCG127950.026.35183746-47
AATCTCG127850.026.20597538-39
TCACACT126400.026.15901430-31
ACACTTG125600.026.02325232-33
GAATCTC127100.025.9674538-39
TCTGCTT123950.025.96726456-57
CACACTT127550.025.9603830-31
CTTGAAA125200.025.84223760-61
Produced by FastQC (version 0.11.2)