FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w3_br.351000000ac99a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w3_br.351000000ac99a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4500027
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC716151.5914348958350695TruSeq Adapter, Index 7 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCA127250.030.22317534-35
AGATCAT129750.029.82380934-35
CTCGTAT129950.029.54218142-43
GTATGCC131800.029.35801744-45
TGCTTGA129300.029.01006158-59
TATGCCG133150.029.00797346-47
ACCAGAT133800.028.92106832-33
CACCAGA135350.028.6249530-31
TCTCGTA134800.028.53178440-41
GCTTGAA132100.028.43110758-59
ATCATCT135400.028.30947536-37
CTGCTTG134000.028.22239156-57
ATGCCGT136850.028.11941346-47
TCATCTC136500.028.03667638-39
TCGTATG137400.027.95768742-43
CGTATGC139900.027.7602944-45
AGTCACC139200.027.6456428-29
TCACCAG139500.027.603230-31
CCGTCTT137800.027.58225650-51
GCCGTCT138250.027.5433848-49
Produced by FastQC (version 0.11.2)