FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w2_ov.351000000ac970.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w2_ov.351000000ac970.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902014
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC386840.6554372795455924TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCAATA86750.022.7128534-35
CACGCCA88050.022.00005730-31
TCACGCC93350.021.43760530-31
CTCGTAT92650.021.38666742-43
ACGCCAA94000.021.06207832-33
CGCCAAT94400.020.54532832-33
AGTCACG97400.020.52183328-29
CCAATAT95350.020.4153234-35
CCAGTCA99300.020.15290626-27
GTCACGC98550.019.77650528-29
GTATGCC99400.019.7368944-45
TATGCCG103250.019.63000546-47
TCTCGTA99100.019.5499140-41
CGTATGC103700.019.4465544-45
ATATCTC103050.019.29653738-39
CAGTCAC101400.019.26731526-27
CCGTCTT104600.019.24532950-51
TCGTATG103150.018.95567142-43
TATCTCG102200.018.94500238-39
GCCGTCT102250.018.87514348-49
Produced by FastQC (version 0.11.2)