FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w2_fb.351000000ac957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w2_fb.351000000ac957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9977658
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC530540.5317279866678132TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGATC128800.022.02370536-37
ACAGTGA132450.021.27292834-35
CACAGTG133800.020.96958732-33
GTGATCT135200.020.91087736-37
GTCACAC139650.020.73691628-29
CACACAG138150.020.61848330-31
ACACAGT140700.020.59918832-33
CGTATGC142450.020.55090744-45
CAGTGAT141000.020.18499434-35
CTCGTAT144800.019.97946742-43
TCGTATG144900.019.94923442-43
AGTCACA146900.019.82659128-29
TCTCGTA146250.019.73593340-41
ATGCCGT148900.019.67092146-47
GTATGCC151000.019.48191344-45
CCGTCTT151900.019.18983850-51
TATGCCG154350.018.96093846-47
GCCGTCT154450.018.82652548-49
CAGTCAC155600.018.61129426-27
TGCTTGA153950.018.33306358-59
Produced by FastQC (version 0.11.2)