FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w2_br.351000000ac93d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w2_br.351000000ac93d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11020347
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC679560.6166412001364385TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCT153900.022.86106736-37
ACTGACC157000.022.769532-33
CTGACCA161350.022.03794332-33
ACCAATC164050.022.02586736-37
CACTGAC164950.021.47062330-31
TGACCAA167850.021.35422134-35
GACCAAT166300.021.31060434-35
GTATGCC171000.020.72594644-45
TATGCCG178850.020.08856646-47
TCACTGA178650.019.9702830-31
CTCGTAT181500.019.83710542-43
TGCTTGA174900.019.8153258-59
AGTCACT183750.019.39016528-29
GTCACTG182750.019.340428-29
CGTATGC187650.019.1408144-45
TCGTATG185900.019.05996342-43
CCAGTCA188550.018.98466726-27
ATGCCGT187950.018.93870246-47
CAATCTC191350.018.75306738-39
CCGTCTT192700.018.4404450-51
Produced by FastQC (version 0.11.2)