FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w1_ov.351000000ac913.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w1_ov.351000000ac913.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6101659
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC359470.5891348566021143TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC71900.026.37911232-33
TAGGCAT72950.026.16223334-35
ACTTAGG73850.026.10044132-33
TTAGGCA76750.024.99062334-35
CACTTAG78450.024.47923730-31
GGCATCT79950.023.52996836-37
AGGCATC82200.023.40603436-37
TCACTTA88350.022.38106530-31
CTCGTAT90750.021.49562542-43
AGTCACT99650.019.91458528-29
TCTCGTA98150.019.8618240-41
GTATGCC99100.019.58371244-45
GCATCTC98950.019.54390138-39
CGTATGC103050.019.27210244-45
TATGCCG103050.019.11593446-47
TCGTATG102200.019.08735842-43
CAGTCAC103450.019.02244626-27
CCAGTCA104750.018.9903226-27
ATGCCGT102250.018.98628646-47
GTCACTT105400.018.53538328-29
Produced by FastQC (version 0.11.2)