FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w1_fb.351000000ac8f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w1_fb.351000000ac8f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8088403
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC477490.5903390323157736TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTC113700.021.651238-39
CGTATGC120550.021.15650244-45
TCGTATG119750.021.04467242-43
AGTCACC120250.020.82578828-29
ATGTATC120050.020.81396736-37
ATGCCGT122300.020.42694946-47
GTATGCC126550.020.39805444-45
CTCGTAT125700.020.25696842-43
CCGTCTT123300.020.19562350-51
GCCGTCT123050.020.02395448-49
CAGTCAC124850.019.94440326-27
CGATGTA125700.019.9228434-35
TCTCGTA126250.019.81473540-41
TATGCCG129050.019.76411846-47
GATGTAT126500.019.64672934-35
ACCGATG130550.019.0008732-33
TGCTTGA130400.018.87791658-59
CCGATGT130550.018.85540832-33
TATCTCG132600.018.69073138-39
CCAGTCA133750.018.67050426-27
Produced by FastQC (version 0.11.2)