FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d3_w1_br.351000000ac8de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d3_w1_br.351000000ac8de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5706300
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC433170.7591083539246096TruSeq Adapter, Index 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC102500.022.53886644-45
TATGCCG105250.022.42522446-47
CTCGTAT104050.022.34909642-43
CGTATGC108950.021.6854644-45
TCGTATG106350.021.64172642-43
TGCTTGA104950.021.48752458-59
GCCGTCT107900.021.20721848-49
CCGTCTT111300.020.94363450-51
ATGCCGT111700.020.7681946-47
CACATCA112650.020.58728630-31
GCTTGAA108750.020.583958-59
ACATCAC114850.020.46161532-33
TCTCGTA112300.020.41769240-41
CCAGTCA113650.020.30152726-27
ACGATCT113150.020.21819736-37
AGTCACA116050.020.18840628-29
CTGCTTG110950.020.17512756-57
CATCACG114450.020.07683632-33
TCACATC117900.020.03295330-31
GTCACAT115450.019.90267228-29
Produced by FastQC (version 0.11.2)