FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_5_ov.351000000acb55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_5_ov.351000000acb55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8880713
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC43650.014.3609327
CTAGACT42350.013.6804188
ATTCTAG55700.012.2842825
CTCTTAT62350.012.03418
GGGGAAT24050.011.8726411
CATTCTA62350.011.5072294
TCTCTTA73750.010.8822237
GGTCATA24600.010.8164114
CCGGGAT26200.010.716721
CGGGAAT44250.010.6471411
CGGGGAT28750.010.5938361
TTCTAGA63900.010.1815796
TAGACTT62700.09.8462039
CGGGCAT25200.09.6312081
CTCGGGT20550.09.0315851
CGCGGGG26350.09.0302661
CTCGGAT45500.08.9949391
GGGGGAT14400.08.9230321
CCGGGGT12955.456968E-128.8196761
CGGGGGT13501.8189894E-128.8128711
Produced by FastQC (version 0.11.2)