FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_5_fb.351000000acb2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_5_fb.351000000acb2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6370211
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC64270.10089147753504554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT40450.016.316868
CCGGGGG6600.015.8619911
TCTAGAC43050.015.5520017
TAGACTT49350.013.8552899
CTAGGAT14450.013.5019041
CATTCTA53150.013.4979054
ATTCTAG55150.012.9226655
ATCTAGG11100.012.4131315
TTCTAGA58850.012.2641496
GGGGATC12200.011.6805152
GGTCATA26550.011.2737584
GGGGAAT13750.011.07455351
GAGGTCA26600.011.0716062
TCTAGGC9900.011.0314597
CCCATAA27250.010.9777769
TAATCCC27750.010.6088679
CTAGGCG10850.010.5032048
TCATTCT75450.010.3900443
CTGGAAT39200.010.1969931
TCTAGGA13050.010.1879746
Produced by FastQC (version 0.11.2)