FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_5_br.351000000acb04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_5_br.351000000acb04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908013
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAAAAGAAACTTTAATAAATATTTTTTCCATAGATGAATGATT138130.1746709318763133No Hit
CTTTCGTACAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCT113820.14392996066142028No Hit
CGAGGTCATAATCCCAATTATATATATACTACAAAATTAAATTATGCTGT109750.13878328222272776No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT104680.13237206362710835TruSeq Adapter, Index 20 (97% over 44bp)
CTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAATTT102810.13000737353365505No Hit
CCCAATTATATATATACTACAAAATTAAATTATGCTGTTATCCCTAAGGT90210.11407416755637606No Hit
CAAATTTATAAAATTTTTTTATAGATAGAAACCGATCTGGCTCACACCGA89450.11311311703711159No Hit
CCTCGATGTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTA86630.10954711379457772No Hit
GTTGAATTAAGATAAATTTTGAAATAAGAATTTTAAATATTTAATCTGTT85610.10825728283451229No Hit
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC81080.10252891592363342No Hit
GTCTTTTCGTCCCATAAATTTATTTAAGTTTTCTTACTTAAAAAATAAAT80740.10209897227027825No Hit
GCCAGATCGGTTTCTATCTATAAAAAAATTTTATAAATTTGTACGAAAGG80130.10132760277455286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCATA43550.031.6440014
AGGTCAT43150.027.2018033
GAGGTCA44100.026.3942972
GTCATAA60250.023.6627395
CGAGGTC58650.020.448971
CTCTTAT65800.020.0636168
TAATCCC68750.019.893459
CGGGGTC17450.017.182411
GGCCTTA36750.016.41656536-37
TCTCTTA82950.016.2589427
GGATAGT40750.015.7668551
ATAATCC91550.015.509698
TGGGTCT15600.015.2540211
TCATAAT94300.014.9566756
CCCAATT79150.014.0703161
GTGGCCT43550.014.00844534-35
CCCATAA77350.013.9365689
GGGTCAT15450.013.84093953
ATCGGTT78550.013.3004646
CCTCGAT80700.013.2692971
Produced by FastQC (version 0.11.2)