FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_1_ov.351000000acae9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_1_ov.351000000acae9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883074
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT51070.17713731940283184TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC13000.020.4578327
CTAGACT14350.017.2094258
ATTCTAG16400.016.2244555
CGGGGAT9000.015.8582531
TTCTAGA19200.014.3463596
CATTCTA19550.013.8526044
TAGACTT18850.013.6049629
GGTCATA8050.012.9846474
CCGGGAT8450.012.3863281
GGGGATT5402.9596777E-812.3140782
CGGGTAT7359.094947E-1212.2982371
CTCTTAT20150.012.0201548
CGGGCAT7601.6370905E-1111.8936891
CGGGAAT14500.011.8116641
GGGGATA6854.765752E-1011.7876182
AGTCACG21200.011.64469128-29
CTGGGAT11550.011.5332751
CGGGGGT3309.0037286E-411.5332741
CCAGTCA23300.011.10445726-27
TCTCTTA22250.011.0991137
Produced by FastQC (version 0.11.2)