FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_1_fb.351000000acadc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_1_fb.351000000acadc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772999
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT99800.26451106931117657TruSeq Adapter, Index 18 (97% over 40bp)
CTTCATTCTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTAC48720.12912804906653833No Hit
CTAGACTTCATTTAAAAGACTATTGATTATGCTACCTTTGTACAGTCAAT38610.10233238863832192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAT12850.029.993291
GGTCATA10450.020.4626564
GTCCGCA25500.019.17684234-35
ATCTAGG8500.019.0082915
CTAGACT26650.018.9256531
TCTAGAC23400.018.8761777
CCGCACA29100.017.22270836-37
TAGACTT30700.016.7102972
TCTAGGA9900.016.3113786
GCACATC30500.016.14258838-39
CACATCT30950.015.90788238-39
CATTCTA29950.015.8660564
CGCACAT31600.015.70981836-37
ATTCTAG31500.015.3876645
TAGGATT27000.014.953882
GTCACGT34500.014.24280428-29
ACGTCCG35500.013.9086532-33
TAATCCC15400.013.569979
CAGTCAC37100.013.56457626-27
TTCTAGA36550.013.3843356
Produced by FastQC (version 0.11.2)