FastQCFastQC Report
Thu 14 Dec 2017
HKC3NBCXY_l01n01_d0_1_br.351000000acab2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKC3NBCXY_l01n01_d0_1_br.351000000acab2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3069744
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC77230.2515844969482797TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC16450.022.2290347
ATTCTAG21100.019.3668735
CTAGACT19700.019.2849968
CATTCTA22800.016.463484
ACCCGTC24900.016.30125632-33
CACCCGT25300.015.94970830-31
TTCTAGA24600.015.8297686
CCGTCCA27200.015.01010934-35
CCCGTCC27200.014.83557232-33
TCACCCG27650.014.50827930-31
TAGACTT27050.014.3960189
GTCACCC28500.014.07557528-29
AGTCACC30750.013.74039428-29
CGTCCAT30950.013.49821434-35
CCAGTCA31700.013.40349626-27
CAGTCAC32650.012.940826-27
GTCCATC31400.012.93732736-37
CCATCTC31850.012.62677138-39
CTGGAAT14700.012.6228551
ACTCCAG35600.012.40187222-23
Produced by FastQC (version 0.11.2)