FastQCFastQC Report
Sat 15 Jun 2019
HK7YLAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7YLAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23939299
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACATG4384491.8315030862014798No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAA4225261.7649890249501459No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCTTTCAGGA4170661.7421813395621983No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACATG4102881.7138680627197982No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGA3995481.66900459365999No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACA3721241.5544481899825053No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACAT3311991.3834949803668017No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTC2945271.2303075374095123No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAAC2700571.128090676339353No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACA2664111.1128604893568521No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAACAT2627151.0974214407865492No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCTTTCAGGAGAAA2510431.048664791730117No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCTTTCAGGAG2446451.0219388629550097No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTCA2395581.0006892850120632No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTCAT2230040.9315393905226714No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTCA2130640.8900177068676907No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGC2128910.8892950457738966No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAG2040790.8524852795397225No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCT1985090.8292180986586115No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTCAT1769510.7391653364620243No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGAGCTC1375700.5746617726776376No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAAGCTATGG1232760.5149524219568835No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAAGCTATG1184810.4949225956866991No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGAAGCTATGGA1131330.47258276025542767No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCAGACCA724420.30260702287063623No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCA577160.2410931080312753No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCAGACC480470.20070345418217972No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCAGAC363310.1517630069284819No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCAGA354070.14790324478590622No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTACCTGCTG333830.1394485277116928No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGTACCTGCTGCC302040.1261691079592598No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTACCTGCTGC271540.11342855110335519No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCTGCTGCCAG271520.11342019663984314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTC794100.0144.503782
ATCGATT791800.0144.435321
CGATCAT607800.0144.347611
CGATAGG678700.0144.316536
GATCGCG636750.0144.275621
TAAGTAG856100.0144.274031
CGATTCC794450.0144.261963
ATCGCGC637050.0144.26132
ACACGAT772350.0144.249453
TCGTTAC659600.0144.196045
ACGATCG678900.0144.188691
GATAACG641550.0144.148015
TCGCATC687150.0144.139547
CACGATC773000.0144.136924
ACAATGG744800.0144.125983
GATCATG608350.0144.121462
TACACGA772550.0144.095492
GATTCCT795950.0144.08044
CTGTATC579200.0144.048954
AACGCAT642050.0144.021188
Produced by FastQC (version 0.11.5)