FastQCFastQC Report
Sat 15 Jun 2019
HK7YLAFXY_n01_CRISPRi_tubeB.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7YLAFXY_n01_CRISPRi_tubeB.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22352136
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262380.11738475463821443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA2476400.0144.899152
TAACAAT1778850.0144.847471
TCGATCG3143000.0144.824131
ATAGGTC1791300.0144.815262
ATCGATT1631050.0144.788251
TAAGTAG2323500.0144.753591
ACGATCG1527650.0144.736651
ATACACG2479300.0144.723081
ACACGAT2479800.0144.702793
ATCGTTA3151250.0144.628344
ACTGTAT1533350.0144.584643
TCGATTC1637350.0144.542242
TCGTTAC3151900.0144.53875
GTCGCAT1792950.0144.48226
TCGATAA1391500.0144.477283
CACGATC2481600.0144.460194
AACGCAT1387950.0144.45958
TCGATAG1530450.0144.446095
ACGCATT1388300.0144.401879
TAGAGTC2330050.0144.376835
Produced by FastQC (version 0.11.5)